Methanogenesis from acetate: Physiology of a thermophilic, acetate-utilizing methanogenic bacterium
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چکیده
منابع مشابه
Methanogenesis from acetate: a nonmethanogenic bacterium from an anaerobic acetate enrichment.
A methanogenic acetate enrichment was initiated by inoculation of an acetate-mineral salts medium with domestic anaerobic digestor sludge and maintained by weekly transfer for 2 years. The enrichment culture contained a Methanosarcina and several obligately anaerobic nonmethanogenic bacteria. These latter organisms formed varying degrees of association with the Methanosarcina, ranging from the ...
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The effects of 2-bromoethanesulfonate, an inhibitor of methanogenesis, on metabolism in sludge from a thermophilic (58 degrees C) anaerobic digestor were studied. It was found from short-term experiments that 1 mumol of 2-bromoethanesulfonate per ml completely inhibited methanogenesis from CH(3)COO, whereas 50 mumol/ml was required for complete inhibition of CO(2) reduction. When 1 mumol of 2-b...
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Methane formation from acetate by resting cells of Methanosarcina barkeri was accompanied by an increase in the intracellular ATP content from 0.9 to 4.0 nmol/mg of protein. Correspondingly, the proton motive force increased to a steady-state level of -120 mV. The transmembrane pH gradient however, was reversed under these conditions and amounted to +20 mV. The addition of the protonophore 3,5,...
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The effects of Mg2+ on thermophilic (55°C) granules grown on acetate in 0.2-liter upflow anaerobic sludge blanket reactors were studied. The methanogens in the granules were identified and counted by using antibody probes and the antigenic fingerprinting method. Packets of large coccoidal cells antigenically related to Methanosarcina thermophila TM-1 were scarce in the absence of Mg2' but incre...
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Function of Ech hydrogenase in ferredoxin-dependent, membrane-bound electron transport in Methanosarcina mazei.
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1985
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1985.tb00755.x